中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (3): 377-382.doi: 10.3969/j.issn.2095-4344.2014.03.008

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

双腔搅拌式生物反应器提高β-磷酸三钙修复关节软骨缺损的效果

黎健伟1,赵  辉1,张晓强2,王  磊1,夏立恒1,金  丹1,王  钢1,余  斌1   

  1. 1南方医科大学南方医院创伤骨科,广东省广州市  510515;2洛阳市正骨医院骨科,河南省洛阳市  471000
  • 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 黎健伟,主治医师,南方医科大学南方医院创伤骨科,广东省广州市 510515
  • 作者简介:黎健伟,男,1979年生,广西壮族自治区南宁市人,汉族,2009年南方医科大学毕业,博士,主治医师,主要从事骨与软骨组织工程研究。
  • 基金资助:

    广东高校优秀青年创新人才培养计划项目(育苗工程LYM11041)

Double-chamber stirred bioreactor improves the repaired effect of beta-tricalcium phosphate on goat knee cartilage defects

Li Jian-wei1, Zhao Hui1, Zhang Xiao-qiang2, Wang Lei1, Xia Li-heng1, Jin Dan1, Wang Gang1, Yu Bin1   

  1. 1 Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 2 Department of Orthopedics, Luoyang Orthopedic-Traumatological Hospital, Luoyang 471000, Henan Province, China
  • Online:2014-01-15 Published:2014-01-15
  • Contact: Li Jian-wei, Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Li Jian-wei, M.D., Attending physician, Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China ljwoven1979@163.com
  • Supported by:

    Foundation for Distinguished Young Talents in Higher Education of Guangdong Province, No. LYM11041

摘要:

背景:前期实验自行研发了一种可进行成骨及成软骨双向诱导分化的双腔搅拌式生物反应器。
目的:探索双腔搅拌式生物反应器的力学刺激能否提高组织工程骨软骨修复山羊膝关节缺损的效果。
方法:取青山羊12只,制作双侧后肢股骨内髁骨软骨缺损,随机分组,实验组与对照组缺损处均植入在双腔搅拌式生物反应器中进行成软骨、成骨诱导2周的自体骨髓间充质干细胞与β-磷酸三钙复合体,不同的是实验组将双腔搅拌式生物反应器置于磁力搅拌仪上给予力学刺激,对照组未给予力学刺激;空白对照组不做处置。植入后12,24周进行大体观察,Masson染色、Ⅱ型胶原免疫组织化学染色和组织学评分。
结果与结论:实验组与对照组均有新生软骨与骨组织生成,实验组修复骨缺损效果明显优于对照组(P < 0.05);空白对照组无新生软骨生成。表明通过双腔搅拌式生物反应器体外培养阶段的力学刺激,可以改善以山羊骨髓间充质干细胞为种子细胞、β-磷酸三钙为支架的组织工程骨软骨复合物对膝关节缺损的修复效果。


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

关键词: 生物材料, 软骨生物材料, 组织工程, 骨髓间充质干细胞, β-磷酸三钙, 生物反应器, 骨软骨, 山羊

Abstract:

BACKGROUND: A preliminary experiment developed a double-chamber stirred bioreactor which can carry out osteogenic and cartilage induction at the same time. 
OBJECTIVE: To explore the effects of double-chamber stirred bioreactor on the repair of goat knee cartilage defects with tissue-engineered cartilage.
METHODS: Twelve goats were selected to make bilateral femoral condyle osteochondral defects models and randomized to three groups: experimental group, implanted with the composites of β-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor under mechanical stimulation; control group, implanted with the composites of β-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor; blank control group, without treatment. After 12 and 24 weeks of implantation, general observation, Masson staining, II collagen immunohistochemical staining and histological scoring were performed.
RESULTS AND CONCLUSION: In the experimental and control groups, new cartilage tissue and bone tissue were visible, but the experimental group showed better repair effects than the control group (P < 0.05). The blank control group had no cartilage formation. These findings indicate that under the mechanical stimulation by the double-chamber stirred bioreactor in vitro, the repair effect of tissue-engineered osteochondral complex on knee joint cartilage defects can be improved.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: biocompatible materials, tissue engineering, bioreactors, stem cells, compomers

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